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Stereological methods for quantitative assessment of hepatic microcirculation
| Citace: |
TONAR, Z., EBERLOVÁ, L., POLÍVKA, J., DAUM, O., WITTER, K., KRÁLÍČKOVÁ, A., GREGOR, T., NEDOROST, L., KOCHOVÁ, P., ROHAN, E., KALUSOVÁ, K., PÁLEK, R., SKÁLA, M., GLANC, D., KRÁLÍČKOVÁ, M., LIŠKA, V. Stereological methods for quantitative assessment of hepatic microcirculation. In Current microscopy contributions to advances in sciences and technology. Badajoz : Formatex Research Center, 2012, s. 737-748. ISBN: 978-84-939843-5-9
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| Druh: | KAPITOLA V KNIZE |
| Jazyk publikace: | eng |
| Anglický název: | Stereological methods for quantitative assessment of hepatic microcirculation |
| Rok vydání: | 2012 |
| Místo konání: | Badajoz |
| Název zdroje: | Formatex Research Center |
| Autoři: | Doc. MUDr. et Mgr. Zbyněk Tonar Ph.D. , MUDr. Lada Eberlová , Ing. et Ing. Jiří Polívka , Doc. MUDr. Ondřej Daum Ph.D. , Dr.med.vet. Kirsti Witter Ph.D. , Anna Králíčková , Ing. Tomáš Gregor PhD. , MUDr. Lukáš Nedorost , Ing. Petra Kochová Ph.D. , Prof. Dr. Ing. Eduard Rohan , Mgr. Kristýna Kalusová , Robert Pálek , Martin Skála , David Glanc , doc. MUDr. Milena Králíčková Ph.D. , MUDr. Václav Liška Ph.D. |
| Abstrakt EN: | This chapter reviews the current knowledge on healthy and pathological hepatic circulation and microcirculation in animal models used in biomedical research and in human histopathology. To demonstrate the clinical importance of this topic, we focus on blood vessels (particularly microvessels) in liver surgery and in hepatic metastasesof colorectal carcinoma. Histological techniques of assessment of healthy and tumourous microvessels are reviewed with focus on the immunohistochemistry on the endothelium, basal lamina and lectin immunohistochemistry. The role of angiogenesis and vascularisation in colorectal cancer and liver metastasis islinked to recent knowledge on proliferation, tumour invasion, metastasis formation, prognostic factors,and scoring systems. In addition tohistopathological methods, the three-dimensional imaging of hepatic microvessels using corrosion casts is shown using X-ray microtomography. Stereological methods are demonstrated to quantify the volume, surface, length, numerical density and tortuosity of the microvessels. |
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